This research was on the Influence of the Healing Ministry on the Psycho-Spiritual Well-Being of the Christians at the Merciful Jesus Sanctuary of Ruhango, Rwanda. The study was guided by James Fowler William’s Theory of Faith Development. It adopted a convergent design, mixed methods. The research objectives of this study are:to explore the Healing Ministry of physical and psychological suffering of Christians at the Merciful Jesus Sanctuary of Ruhango, Rwanda; to examine the effects of the Healing Ministry on improvement of life of Christians at the Merciful Jesus Sanctuary of Ruhango, Rwanda; to find outthe influence of the Healing Ministry on Psycho-spiritual well-being of the Christians at the Merciful Jesus Sanctuary of Ruhango, Rwanda; to determine at the possible ways to increase the well-being and faith of the Christians at the Merciful Jesus Sanctuary of Ruhango, Rwanda.The target population was102 Christians and among them, 81 participants were selected and composed the sample size. The data collection was done through a questionnaire, guided interview and focus group. The analysis of the results was done in relation to the research objectives. For quantitative questions, the researcher used frequencies, tables and diagrams. Quantitative data was analysed using the Statistical Package for Social Sciences IBM (SPSS) version 26. The qualitative data was drawn from the guide interview and focus group. Both were presented in narrative form using the research objective themes. The findings showed that the Healing Ministry influences positively the psycho-spiritual well-being of Christians in the Sanctuary of Ruhango and it increases their faith.They indicated its importance in the life’s journey of human beings.